GSP
Quick Navigator

Search Site

Unix VPS
A - Starter
B - Basic
C - Preferred
D - Commercial
MPS - Dedicated
Previous VPSs
* Sign Up! *

Support
Contact Us
Online Help
Handbooks
Domain Status
Man Pages

FAQ
Virtual Servers
Pricing
Billing
Technical

Network
Facilities
Connectivity
Topology Map

Miscellaneous
Server Agreement
Year 2038
Credits
 

USA Flag

 

 

Man Pages


Manual Reference Pages  -  ALIGN (1)

NAME

align - compute the global alignment of two protein or DNA sequences

align0 - compute the global alignment of two protein or DNA sequences without penalizing for end-gaps

CONTENTS

Synopsis
Description
Options
Examples
Author

SYNOPSIS

align [ -f # -g # -O filename -m # -s SMATRIX -w # ] sequence-file-1 sequence-file-2

DESCRIPTION

align produces an optimal global alignment between two protein or DNA sequences. align will automatically decide whether the query sequence is DNA or protein by reading the query sequence as protein and determining whether the ‘amino-acid composition’ is more than 85% A+C+G+T. align uses a modification of the algorithm described by E. Myers and W. Miller in "Optimal Alignments in Linear Space" CABIOS (1988) 4:11-17. The program can be invoked either with command line arguments or in interactive mode.

align weights end gaps, so that an alignment of the form



-----MACF
SRTKIMACF


will have a higher score than:


MACF
MACF



align0 uses the same algorithm, but does not weight end gaps. Sometimes this can have surprising effects.

align and align0 use the standard fasta format sequence file. Lines beginning with ’>’ or ’;’ are considered comments and ignored; sequences can be upper or lower case, blanks,tabs and unrecognizable characters are ignored. align expects sequences to use the single letter amino acid codes, see protcodes(1) .

OPTIONS

align can be directed to change the scoring matrix and output format by entering options on the command line (preceeded by a ‘-’ or ‘/’ for MS-DOS). All of the options should preceed the file name arguments. Alternately, these options can be changed by setting environment variables. The options and environment variables are:
-f # Penalty for the first residue in a gap (-12 by default).
-g # Penalty for additional residues in a gap (-2 by default).
-O filename
  Sends copy of results to "filename".
-m # (MARKX) =1,2,3. Alternate display of matches and mismatches in alignments. MARKX=1 uses ":","."," ", for identities, consevative replacements, and non-conservative replacements, respectively. MARKX=2 uses " ","x", and "X". MARKX=3 does not show the second sequence, but uses the second alignment line to display matches with a "." for identity, or with the mismatched residue for mismatches. MARKX=3 is useful for aligning large numbers of similar sequences.
-s str (SMATRIX) the filename of an alternative scoring matrix file or "250" to use the PAM250 matrix.
-w # (LINLEN) output line length for sequence alignments. (normally 60, can be set up to 200).

EXAMPLES

(1) align musplfm.aa lcbo.aa
Compare the amino acid sequence in the file musplfm.aa with the amino acid sequence in the file lcbo.aa Each sequence should be in the form:


>LCBO bovine preprolactin
WILLLSQ ...


(2) align -w 80 musplfm.aa lcbo.aa > musplfm.aln
Compare the amino acid sequence in the file musplfm.aa with the sequences in the file lcbo.aa Show both sequences with 80 residues on each output line and write the output to the file musplfm.aln .
(3) align
Run the align program in interactive mode. The program will prompt for the file name for the first sequence and the second sequence.

SEE ALSO

rdf2(1),protcodes(5), dnacodes(5)

AUTHOR

Bill Pearson
wrp@virginia.EDU
Search for    or go to Top of page |  Section 1 |  Main Index


--> ALIGN (1) local

Powered by GSP Visit the GSP FreeBSD Man Page Interface.
Output converted with manServer 1.07.