Manual Reference Pages - BP_FLANKS.PL (1)
bp_flanks - finding flanking sequences for a variant in a sequence position
bp_flanks --position POS [-p POS ...] [--flanklen INT]
accession | filename
This script allows you to extract a subsequence around a region of
interest from an existing sequence. The output if fasta formatted
sequence entry where the header line contains additional information
about the location.
The script takes one unnamed argument which be either a file name in
the local file system or a nucleotide sequence accession number.
-p Position uses simple nucleotide sequence feature table
--position notation to define the region of interest, typically a
SNP or microsatellite repeat around which the flanks are
There can be more than one position option or you can
give a comma separated list to one position option.
The format of a position is:
[id:] int | range | in-between [-]
The optional id is the name you want to call the new
sequence. If it not given in joins running number to the
entry name with an underscore.
The position is either a point (e.g. 234), a range (e.g
250..300) or insertion point between nucleotides
If the position is not completely within the source
sequence the output sequence will be truncated and it
will print a warning.
The optional hyphen [-] at the end of the position
indicates that that you want the retrieved sequence to be
in the opposite strand.
-f Defaults to 100. This is the length of the nucleotides
--flanklen sequence retrieved on both sides of the given position.
If the source file does not contain
The output is a fasta formatted entry where the description file
contains tag=value pairs for information about where in the original
sequence the subsequence was taken.
The ID of the fasta entry is the name given at the command line joined
by hyphen to the filename or accesion of the source sequence. If no id
is given a series of consequtive integers is used.
The tag=value pairs are:
The sequence highlights the allele variant position by showing it in
upper case and rest of the sequence in lower case characters.
position in the source file
strand of the sequence compared to the source sequence
position of the region of interest in the current entry.
The tag is the same as used by dbSNP@NCBI
% bp_flanks ~/seq/ar.embl
>1_/HOME/HEIKKI/SEQ/AR.EMBL oripos=100 strand=1 allelepos=100
The input files are assumed to be in EMBL format and the sequences are
retrieved only from the EMB database. Make this more generic and use
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to the
Bioperl mailing lists Your participation is much appreciated.
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the bugs and their resolution. Bug reports can be submitted via the
AUTHOR - Heikki Lehvaslaiho
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