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Man Pages


Manual Reference Pages  -  TABIX (1)

NAME

bgzip - Block compression/decompression utility

tabix - Generic indexer for TAB-delimited genome position files

CONTENTS

Synopsis
Description
Indexing Options
Querying And Other Options
Example
Notes
Author
See Also

SYNOPSIS

bgzip [-cdhB] [-b virtualOffset] [-s size] [file]

tabix [-0lf] [-p gff|bed|sam|vcf] [-s seqCol] [-b begCol] [-e endCol] [-S lineSkip] [-c metaChar] in.tab.bgz [region1 [region2 [...]]]

DESCRIPTION

Tabix indexes a TAB-delimited genome position file in.tab.bgz and creates an index file ( in.tab.bgz.tbi or in.tab.bgz.csi ) when region is absent from the command-line. The input data file must be position sorted and compressed by bgzip which has a gzip(1) like interface. After indexing, tabix is able to quickly retrieve data lines overlapping regions specified in the format "chr:beginPos-endPos". Fast data retrieval also works over network if URI is given as a file name and in this case the index file will be downloaded if it is not present locally.

INDEXING OPTIONS

-0, --zero-based
  Specify that the position in the data file is 0-based (e.g. UCSC files) rather than 1-based.
-b, --begin INT
  Column of start chromosomal position. [4]
-c, --comment CHAR
  Skip lines started with character CHAR. [#]
-C, --csi Skip lines started with character CHAR. [#]
-e, --end INT
  Column of end chromosomal position. The end column can be the same as the start column. [5]
-f, --force
  Force to overwrite the index file if it is present.
-m, --min-shiftINT
  set minimal interval size for CSI indices to 2^INT [14]
-p, --preset STR
  Input format for indexing. Valid values are: gff, bed, sam, vcf. This option should not be applied together with any of -s, -b, -e, -c and -0; it is not used for data retrieval because this setting is stored in the index file. [gff]
-s, --sequence INT
  Column of sequence name. Option -s, -b, -e, -S, -c and -0 are all stored in the index file and thus not used in data retrieval. [1]
-S, --skip-lines INT
  Skip first INT lines in the data file. [0]

QUERYING AND OTHER OPTIONS

-h, --print-header
  Print also the header/meta lines.
-H, --only-header
  Print only the header/meta lines.
-l, --list-chroms
  List the sequence names stored in the index file.
-r, --reheader FILE
  Replace the header with the content of FILE
-R, --regions FILE
  Restrict to regions listed in the FILE. The FILE can be BED file (requires .bed, .bed.gz, .bed.bgz file name extension) or a TAB-delimited file with CHROM, POS, and, optionally, POS_TO columns, where positions are 1-based and inclusive. When this option is in use, the input file may not be sorted. regions.
-T, --targets FILE
  Similar to -R but the entire input will be read sequentially and regions not listed in FILE will be skipped.

EXAMPLE

(grep ^"#" in.gff; grep -v ^"#" in.gff | sort -k1,1 -k4,4n) | bgzip > sorted.gff.gz;

tabix -p gff sorted.gff.gz;

tabix sorted.gff.gz chr1:10,000,000-20,000,000;

NOTES

It is straightforward to achieve overlap queries using the standard B-tree index (with or without binning) implemented in all SQL databases, or the R-tree index in PostgreSQL and Oracle. But there are still many reasons to use tabix. Firstly, tabix directly works with a lot of widely used TAB-delimited formats such as GFF/GTF and BED. We do not need to design database schema or specialized binary formats. Data do not need to be duplicated in different formats, either. Secondly, tabix works on compressed data files while most SQL databases do not. The GenCode annotation GTF can be compressed down to 4%. Thirdly, tabix is fast. The same indexing algorithm is known to work efficiently for an alignment with a few billion short reads. SQL databases probably cannot easily handle data at this scale. Last but not the least, tabix supports remote data retrieval. One can put the data file and the index at an FTP or HTTP server, and other users or even web services will be able to get a slice without downloading the entire file.

AUTHOR

Tabix was written by Heng Li. The BGZF library was originally implemented by Bob Handsaker and modified by Heng Li for remote file access and in-memory caching.

SEE ALSO

samtools(1)

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htslib-1.3 TABIX (1) 15 December 2015

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