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Manual Reference Pages  -  BIO::DB::TFBS::TRANSFAC_PRO (3)

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NAME

Bio::DB::TFBS::transfac_pro - An implementation of Bio::DB::TFBS which uses local flat files for transfac pro

CONTENTS

SYNOPSIS



  use Bio::DB::Taxonomy;

  my $db = new Bio::DB::Taxonomy(-source => transfac_pro
                                 -dat_dir => $directory);

  # were interested in the gene P5
  my ($gene_id) = $db->get_gene_ids(-name => P5); # G000001

  # we want all the transcription factors that bind to our gene
  my @factor_ids = $db->get_factor_ids(-gene => $gene_id);

  # get info about those TFs
  foreach my $factor_id (@factor_ids) {
    my $factor = $db->get_factor($factor_id);
    my $name = $factor->universal_name;
    # etc. - see Bio::Map::TranscriptionFactor, eg. find out where it binds
  }

  # get a matrix
  my $matrix = $db->get_matrix(M00001);

  # get a binding site sequence
  my $seq = $db->get_site(R00001);



DESCRIPTION

This is an implementation which uses local flat files and the DB_File module RECNO data structures to manage a local copy of the Transfac Pro TFBS database.

Required database files require a license which can be obtained via http://www.biobase-international.com/pages/index.php?id=170

Within the linux installation tarball you will find a cgibin tar ball, and inside that is a data directory containing the .dat files needed by this module. Point to that data directory with -dat_dir

FEEDBACK

    Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.



  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists



    Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

    Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:



  https://github.com/bioperl/bioperl-live/issues



AUTHOR - Sendu Bala

Email bix@sendu.me.uk

CONTRIBUTORS

Based on Bio::DB::Taxonomy::flatfile by Jason Stajich

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

    new



 Title   : new
 Usage   : my $obj = new Bio::DB::TFBS::transfac_pro();
 Function: Builds a new Bio::DB::TFBS::transfac_pro object
 Returns : an instance of Bio::DB::TTFBS::transfac_pro
 Args    : -dat_dir   => name of directory where Transfac Pro .dat files
                         (required to initially build indexes)
           -tax_db    => Bio::DB::Taxonomy object, used when initially building
                         indexes, gives better results for species information
                         but not required.
           -index_dir => name of directory where index files should be created
                         or already exist. (defaults to -dat_dir, required if
                         -dat_dir not supplied)
           -force     => 1 replace current indexes even if they exist



    Bio::DB::TFBS Interface implementation

    get_reference



 Title   : get_reference
 Usage   : my $ref = $obj->get_reference($id);
 Function: Get a literature reference.
 Returns : Bio::Annotation::Reference
 Args    : string - a reference id (RE...)



    get_genemap



 Title   : get_genemap
 Usage   : my $map = $obj->get_genemap($id);
 Function: Get a GeneMap for a gene.
 Returns : Bio::Map::GeneMap
 Args    : string - a gene id (G...), and optionally int (number of bp
           upstream)



    get_seq



 Title   : get_seq
 Usage   : my $seq = $obj->get_seq($id);
 Function: Get the sequence of a site. The sequence will be annotated with the
           the tags relative_start, relative_end, relative_type and
           relative_to.
 Returns : Bio::Seq
 Args    : string - a site id (R...)



    get_fragment



 Title   : get_fragment
 Usage   : my $seq = $obj->get_fragment($id);
 Function: Get the sequence of a fragment.
 Returns : Bio::Seq
 Args    : string - a site id (FR...)



    get_matrix



 Title   : get_matrix
 Usage   : my $matrix = $obj->get_matrix($id);
 Function: Get a matrix that describes a binding site.
 Returns : Bio::Matrix::PSM::SiteMatrix
 Args    : string - a matrix id (M...), optionally a sequence string from
           which base frequencies will be calcualted for the matrix model
           (default 0.25 each)



    get_aln



 Title   : get_aln
 Usage   : my $aln = $obj->get_aln($id);
 Function: Get the alignment that was used to generate a matrix. Each sequence
           in the alignment will have an accession_number corresponding to the
           Transfac site id, and id() based on that but unique within the
           alignment.
 Returns : Bio::SimpleAlign
 Args    : string - a matrix id (M...), optionally true to, when a matrix
           lists no sequences, search for sequences via the matrixs factors,
           picking the sites that best match the matrix



    get_factor



 Title   : get_factor
 Usage   : my $factor = $obj->get_factor($id);
 Function: Get the details of a transcription factor.
 Returns : Bio::Map::TranscriptionFactor
 Args    : string - a factor id (T...)



    get_reference_ids



 Title   : get_reference_ids
 Usage   : my @ids = $obj->get_reference_ids(-key => $value);
 Function: Get all the reference ids that are associated with the supplied
           args.
 Returns : list of strings (ids)
 Args    : -key => value, where value is a string id, and key is one of:
           -pubmed -site -gene -matrix -factor



    get_site_ids



 Title   : get_site_ids
 Usage   : my @ids = $obj->get_site_ids(-key => $value);
 Function: Get all the site ids that are associated with the supplied
           args.
 Returns : list of strings (ids)
 Args    : -key => value, where value is a string id, and key is one of:
           -id -species -gene -matrix -factor -reference



    get_matrix_ids



 Title   : get_matrix_ids
 Usage   : my @ids = $obj->get_matrix_ids(-key => $value);
 Function: Get all the matrix ids that are associated with the supplied
           args.
 Returns : list of strings (ids)
 Args    : -key => value, where value is a string id, and key is one of:
           -id -name -site -factor -reference



    get_factor_ids



 Title   : get_factor_ids
 Usage   : my @ids = $obj->get_factor_ids(-key => $value);
 Function: Get all the factor ids that are associated with the supplied
           args.
 Returns : list of strings (ids)
 Args    : -key => value, where value is a string id, and key is one of:
           -id -name -species -interactors -gene -matrix -site -reference
           NB: -gene only gets factor ids for genes that encode factors



    get_fragment_ids



 Title   : get_fragment_ids
 Usage   : my @ids = $obj->get_fragment_ids(-key => $value);
 Function: Get all the fragment ids that are associated with the supplied
           args.
 Returns : list of strings (ids)
 Args    : -key => value, where value is a string id, and key is one of:
           -id -species -gene -factor -reference



    Helper methods

    index_directory



 Title   : index_directory
 Funtion : Get/set the location that index files are stored. (this module
           will index the supplied database)
 Usage   : $obj->index_directory($newval)
 Returns : value of index_directory (a scalar)
 Args    : on set, new value (a scalar or undef, optional)



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perl v5.20.3 BIO::DB::TFBS::TRANSFAC_PRO (3) 2016-04-05

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