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Man Pages


Manual Reference Pages  -  BIO::TOOLS::INFERNAL (3)

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NAME

Bio::Tools::Infernal - A parser for Infernal output

CONTENTS

SYNOPSIS



  use Bio::Tools::Infernal;
  my $parser = Bio::Tools::Infernal->new(-file => $rna_output,
                                        -motiftag => misc_binding
                                        -desctag => Lysine riboswitch,
                                        -cm    => RF00168,
                                        -rfam  =>  RF00168,
                                        -minscore => 20);
  #parse the results, get a Bio::SeqFeature::FeaturePair
  while( my $motif = $parser->next_prediction) {
    # do something here
  }



DESCRIPTION

This is a highly experimental parser for Infernal output from the cmsearch program. At some point it is anticipated that this will morph into a proper SearchIO parser, along with the related RNAMotif and ERPIN tools.

The Infernal suite of programs are used for generating RNA CM (covariance models) and searching sequences using CMs to locate potentially similar structures. The program is under active development; it is anticiapted that this will support the latest version available.

This parser has been tested and is capable of parsing Infernal 0.7 and 0.71 output. However, future Infernal versions may break parsing as the output is constantly evolving, so keep an eye on this space for additional notes.

Currently data is parsed into a Bio::SeqFeature::FeaturePair object, consisting of a query (the covariance model) and the hit (sequence searched).

Model data is accessible via the following:



  Data            SeqFeature::FeaturePair         Note
  --------------------------------------------------------------------------
  primary tag     $sf->primary_tag                Rfam ID (if passed to new())
  start           $sf->start                      Based on CM length
  end             $sf->end                        Based on CM length
  score           $sf->score                      Bit score
  strand          $sf->strand                     0 (CM does not have a strand)
  seqid           $sf->seq_id                     Rfam ID (if passed to new())
  display name    $sf->feature1->display_name     CM name (if passed to new())
  source          $sf->feature1->source tag      Infernal followed by version



Hit data is accessible via the following:



  Data            SeqFeature::FeaturePair         Note
  ------------------------------------------------------------------
  start           $sf->hstart
  end             $sf->hend
  score(bits)     $sf->hscore
  strand          $sf->hstrand
  seqid           $sf->hseqid
  Primary Tag     $sf->hprimary_tag
  Source Tag      $sf->hsource_tag



Added FeaturePair tags are :



   secstructure - entire description line (in case the regex used for
                  sequence ID doesnt adequately catch the name
   model        - name of the descriptor file (may include path to file)
   midline      - contains structural information from the descriptor
                  used as a query
   hit          - sequence of motif, separated by spaces according to
                  matches to the structure in the descriptor (in
                  SecStructure).
   seqname      - raw sequence name (for downstream parsing if needed)



An additional parameter (’minscore’) is added due to the huge number of spurious hits generated by cmsearch. This screens data, only building and returning objects when a minimal bitscore is present.

See t/rnamotif.t for example usage.

FEEDBACK

    Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.



  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists



    Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

    Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:



  https://github.com/bioperl/bioperl-live/issues



AUTHOR - Chris Fields

Email cjfields-at-uiuc-dot-edu

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

    new



 Title   : new
 Usage   : my $obj = Bio::Tools::Infernal->new();
 Function: Builds a new Bio::Tools::Infernal object
 Returns : an instance of Bio::Tools::Infernal
 Args    : -fh/-file  - for input filehandle/filename
           -motiftag  - primary tag used in gene features (default misc_binding)
           -desctag   - tag used for display_name name (default infernal)
           -srctag    - source tag used in all features (default Infernal)
           -rfam      - Rfam id number
           -cm        - covariance model used in analysis (may be same as rfam #)
           -minscore  - minimum score (simple screener, since Infernal generates
                        a ton of spurious hits)
           -version   - Infernal program version



    motif_tag



 Title   : motif_tag
 Usage   : $obj->motif_tag($newval)
 Function: Get/Set the value used for motif_tag, which is used for setting the
           primary_tag.
           Default is misc_binding as set by the global $MotifTag.
           misc_binding is used here because a conserved RNA motif is capable
           of binding proteins (regulatory proteins), antisense RNA (siRNA),
           small molecules (riboswitches), or nothing at all (tRNA,
           terminators, etc.).  It is recommended that this be changed to other
           tags (misc_RNA, protein_binding, tRNA, etc.) where appropriate.
           For more information, see:
           http://www.ncbi.nlm.nih.gov/collab/FT/index.html
 Returns : value of motif_tag (a scalar)
 Args    : on set, new value (a scalar or undef, optional)



    source_tag



 Title   : source_tag
 Usage   : $obj->source_tag($newval)
 Function: Get/Set the value used for the source_tag.
           Default is Infernal as set by the global $SrcTag
 Returns : value of source_tag (a scalar)
 Args    : on set, new value (a scalar or undef, optional)



    desc_tag



 Title   : desc_tag
 Usage   : $obj->desc_tag($newval)
 Function: Get/Set the value used for the query motif.  This will be placed in
           the tag -display_name.  Default is infernal as set by the global
           $DescTag.  Use this to manually set the descriptor (motif searched for).
           Since there is no way for this module to tell what the motif is from the
           name of the descriptor file or the Infernal output, this should
           be set every time an Infernal object is instantiated for clarity
 Returns : value of exon_tag (a scalar)
 Args    : on set, new value (a scalar or undef, optional)



    covariance_model



 Title   : covariance_model
 Usage   : $obj->covariance_model($newval)
 Function: Get/Set the value used for the covariance model used in the analysis.
 Returns : value of exon_tag (a scalar)
 Args    : on set, new value (a scalar or undef, optional)



    rfam



 Title   : rfam
 Usage   : $obj->rfam($newval)
 Function: Get/Set the Rfam accession number
 Returns : value of exon_tag (a scalar)
 Args    : on set, new value (a scalar or undef, optional)



    minscore



 Title   : minscore
 Usage   : $obj->minscore($newval)
 Function: Get/Set the minimum score threshold for generating SeqFeatures
 Returns : value of exon_tag (a scalar)
 Args    : on set, new value (a scalar or undef, optional)



    program_version



 Title   : program_version
 Usage   : $obj->program_version($newval)
 Function: Get/Set the Infernal program version
 Returns : value of exon_tag (a scalar)
 Args    : on set, new value (a scalar or undef, optional)
           Note: this is set to $DEFAULT_VERSION by, um, default



    analysis_method



 Usage     : $obj->analysis_method();
 Purpose   : Inherited method. Overridden to ensure that the name matches
             /Infernal/i.
 Returns   : String
 Argument  : n/a



    next_feature



 Title   : next_feature
 Usage   : while($gene = $obj->next_feature()) {
                  # do something
           }
 Function: Returns the next gene structure prediction of the RNAMotif result
           file. Call this method repeatedly until FALSE is returned.
           The returned object is actually a SeqFeatureI implementing object.
           This method is required for classes implementing the
           SeqAnalysisParserI interface, and is merely an alias for
           next_prediction() at present.
 Returns : A Bio::Tools::Prediction::Gene object.
 Args    : None (at present)



    next_prediction



 Title   : next_prediction
 Usage   : while($gene = $obj->next_prediction()) {
                  # do something
           }
 Function: Returns the next gene structure prediction of the RNAMotif result
           file. Call this method repeatedly until FALSE is returned.
 Returns : A Bio::SeqFeature::Generic object
 Args    : None (at present)



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