Use Bio::DB::GFF->new() to construct new instances of this class.
Three named arguments are recommended:
-adaptor Set to "memory" to create an instance of this class.
-gff Read the indicated file or directory of .gff file.
-fasta Read the indicated file or directory of fasta files.
-dir Indicates a directory containing .gff and .fa files
If you use the -dir option and the indicated directory is writable by
the current process, then this library will create a FASTA file index
that greatly diminishes the memory usage of this module.
Alternatively you may create an empty in-memory object using just the
-adaptor=>memory argument and then call the load_gff_file() and
load_fasta_file() methods to load GFF and/or sequence
information. This is recommended in CGI/mod_perl/fastCGI environments
because these methods do not modify STDIN, unlike the constructor.