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Manual Reference Pages  -  BIO::SEQEVOLUTION::FACTORY (3)

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Bio::SeqEvolution::Factory - Factory object to instantiate sequence evolving classes



    # not an instantiable class


This is the factory class that can be used to call for a specific model to mutate a sequence.

Bio::SeqEvolution::DNAPoint is the default for nucleotide sequences and the only implementation at this point.


    Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.                  - General discussion  - About the mailing lists


Please direct usage questions or support issues to the mailing list:

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

    Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:


  Heikki Lehvaslaiho E<lt>heikki at bioperl dot orgE<gt>


Additional contributor’s names and emails here


The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _


  Title   : new
  Usage   : my $obj = Bio::SeqEvolution::Factory->new();
  Function: Builds a new Bio:SeqEvolution::EvolutionI object
  Returns : Bio:SeqEvolution::EvolutionI object
  Args    : -type           => class name

See <Bio:SeqEvolution::EvolutionI>


 Title   : _load_format_module
 Usage   : *INTERNAL SeqIO stuff*
 Function: Loads up (like use) a module at run time on demand
 Example :
 Returns :
 Args    :


 Title   : type
 Usage   : $obj->type($newval)
 Function: Set used evolution model. It is set by giving a
           valid Bio::SeqEvolution::* class name
 Returns : value of type
 Args    : newvalue (optional)

Defaults to Bio::SeqEvolution::DNAPoint.

mutation counters

The next three methods set a value to limit the number of mutations introduced the the input sequence.


 Title   : identity
 Usage   : $obj->identity($newval)
 Function: Set the desired identity between original and mutated sequence
 Returns : value of identity
 Args    : newvalue (optional)


 Title   : pam
 Usage   : $obj->pam($newval)
 Function: Set the wanted Percentage of Accepted Mutations, PAM
 Returns : value of PAM
 Args    : newvalue (optional)

When you are measuring sequence divergence, PAM needs to be estimated. When you are generating sequences, PAM is simply the count of mutations introduced to the reference sequence normalised to the original sequence length.


 Title   : mutation_count
 Usage   : $obj->mutation_count($newval)
 Function: Set the number of wanted mutations to the sequence
 Returns : value of mutation_count
 Args    : newvalue (optional)


 Title   : seq
 Usage   : $obj->seq($newval)
 Function: Set the sequence object for the original sequence
 Returns : The sequence object
 Args    : newvalue (optional)

Setting this will reset mutation and generated mutation counters.


 Title   : seq_type
 Usage   : $obj->seq_type($newval)
 Function: Set the returned seq_type to one needed
 Returns : value of seq_type
 Args    : newvalue (optional)

Defaults to Bio::PrimarySeq.


 Title   : get_mutation_counter
 Usage   : $obj->get_mutation_counter()
 Function: Get the count of sequences created
 Returns : value of counter
 Args    : -


 Title   : reset_mutation_counter
 Usage   : $obj->reset_mutation_counter()
 Function: Resert the counter of mutations
 Returns : value of counter
 Args    : -


 Title   : get_sequence_counter
 Usage   : $obj->get_sequence_counter()
 Function: Get the count of sequences created
 Returns : value of counter
 Args    : -


 Title   : reset_sequence_counter
 Usage   : $obj->reset_sequence_counter()
 Function: Resert the counter of sequences created
 Returns : value of counter
 Args    : -

This is called when ever mutated sequences are reassigned new values using methods seq() and mutated_seq(). As a side affect, this method also recreates the intermal alignment that is used to calculate the sequence identity.


 Title   : each_seq
 Usage   : $obj->each_seq($int)
 Returns : an array of sequences mutated from the reference sequence
           according to evolutionary parameters given
 Args    : -


  Title   : each_mutation
  Usage   : $obj->each_mutation
  Function: return the mutations leading to the last generated
            sequence in objects
  Returns : an array of Bio::Variation::DNAMutation objects
  Args    : optional argument to return an array of  stringified names

Internal methods


 Title   : _increase_mutation_counter
 Usage   : $obj->_increase_mutation_counter()
 Function: Internal method to increase the counter of mutations performed
 Returns : value of counter
 Args    : -


 Title   : _increase_sequence_counter
 Usage   : $obj->_increase_sequence_counter()
 Function: Internal method to increase the counter of sequences created
 Returns : value of counter
 Args    : -

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