NAME

Bio::SearchIO::gmap_f9 - Event generator for parsing gmap reports (Z format)

SYNOPSIS

   # Do not use this object directly - it is used as part of the
   # Bio::SearchIO system.
    use Bio::SearchIO;
    my $searchio = Bio::SearchIO->new(-format => 'gmap',
                                      -file   => 't/data/her2.gmapz');
    while( my $result = $searchio->next_result ) {
        while( my $hit = $result->next_hit ) {
            while( my $hsp = $hit->next_hsp ) {
                # ...
            }
        }
    }

DESCRIPTION

This object encapsulated the necessary methods for generating events suitable for building Bio::Search objects from a GMAP "compressed" report (from gmap run with -Z flag) Read the Bio::SearchIO for more information about how to use this.

REVERSE STRAND AND BIOPERL COORDINATES

I believe that I'm doing the correct thing when reporting hits on the negative strand of the genome. In particular, I've compared the "exons" this code generates with the set returned by ncbi's megablast web service. NCBI's hsp's are ordered differently and have a different genomic location (off by ~18,000,000 bases, padding?) but the starts, ends, and lengths were similar and my strand handling matches theirs. E.g.

   CDNA                            GENOME
 start  end    strand   start           end             strand

blast
1913 2989 1 86236731 86237808 -1
1 475 1 86260509 86260983 -1
1510 1727 1 86240259 86240476 -1
841 989 1 86243034 86243182 -1
1381 1514 1 86240630 86240763 -1
989 1122 1 86242457 86242590 -1
599 729 1 86247470 86247600 -1
473 608 1 86259972 86260107 -1
1255 1382 1 86240837 86240964 -1
730 842 1 86244040 86244152 -1
1813 1921 1 86238123 86238231 -1
1725 1814 1 86239747 86239836 -1
1167 1256 1 86241294 86241383 -1
1120 1188 1 86242319 86242387 -1

gmap
1 475 1 104330509 104330983 -1
476 600 1 104329980 104330104 -1
601 729 1 104317470 104317598 -1
730 841 1 104314041 104314152 -1
842 989 1 104313034 104313181 -1
990 1121 1 104312458 104312589 -1
1122 1187 1 104312320 104312385 -1
1188 1256 1 104311294 104311362 -1
1257 1382 1 104310837 104310962 -1
1383 1511 1 104310633 104310761 -1
1512 1726 1 104310260 104310474 -1
1727 1814 1 104309747 104309834 -1
1815 1917 1 104308127 104308229 -1
1918 2989 1 104306731 104307802 -1

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  https://github.com/bioperl/bioperl-live/issues

AUTHOR - George Hartzell

Email hartzell@alerce.com

CONTRIBUTORS

Additional contributors names and emails here

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with an underscore (_).

next_result

 Title   : next_result
 Usage   : $result = stream->next_result
 Function: Reads the next ResultI object from the stream and returns it.
 Returns : A Bio::Search::Result::ResultI object
 Args    : n/a