|
NAMEBio::SearchIO::infernal - SearchIO-based Infernal parser SYNOPSIS my $parser = Bio::SearchIO->new(-format => 'infernal',
-file => 'purine.inf');
while( my $result = $parser->next_result ) {
# general result info, such as model used, Infernal version
while( my $hit = $result->next_hit ) {
while( my $hsp = $hit->next_hsp ) {
# ...
}
}
}
DESCRIPTIONThis is a SearchIO-based parser for Infernal output from the cmsearch program. It currently parses cmsearch output for Infernal versions 0.7-1.1; older versions may work but will not be supported. The latest version of Infernal is 1.1. The output has changed substantially relative to version 1.0. Versions 1.x are stable releases (and output has stabilized) therefore it is highly recommended that users upgrade to using the latest Infernal release. Support for the older pre-v.1 developer releases will be dropped for future core 1.6 releases. FEEDBACKMailing ListsUser feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists SupportPlease direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting BugsReport bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: https://github.com/bioperl/bioperl-live/issues AUTHOR - Chris FieldsEmail cjfields-at-uiuc-dot-edu CONTRIBUTORSJeffrey Barrick, Michigan State University Paul Cantalupo, University of Pittsburgh APPENDIXThe rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ new Title : new
Usage : my $obj = Bio::SearchIO::infernal->new();
Function: Builds a new Bio::SearchIO::infernal object
Returns : Bio::SearchIO::infernal
Args : -fh/-file => cmsearch (infernal) filename
-format => 'infernal'
-model => query model (Rfam ID) (default undef)
-database => database name (default undef)
-query_acc => query accession, eg. Rfam accession RF####
-query_desc => query description, eg. Rfam description
-hsp_minscore => minimum HSP score cutoff
-convert_meta => boolean, set to convert meta string to simple WUSS format
-symbols => hash ref of structure symbols to use
(default symbols in %STRUCTURE_SYMBOLS hash)
next_resultTitle : next_result Usage : my $hit = $searchio->next_result; Function: Returns the next Result from a search Returns : Bio::Search::Result::ResultI object Args : none start_elementTitle : start_element Usage : $eventgenerator->start_element Function: Handles a start element event Returns : none Args : hashref with at least 2 keys 'Data' and 'Name' end_elementTitle : start_element Usage : $eventgenerator->end_element Function: Handles an end element event Returns : none Args : hashref with at least 2 keys, 'Data' and 'Name' element Title : element
Usage : $eventhandler->element({'Name' => $name, 'Data' => $str});
Function: Convenience method that calls start_element, characters, end_element
Returns : none
Args : Hash ref with the keys 'Name' and 'Data'
element_hash Title : element
Usage : $eventhandler->element_hash({'Hsp_hit-from' => $start,
'Hsp_hit-to' => $end,
'Hsp_score' => $lastscore});
Function: Convenience method that takes multiple simple data elements and
maps to appropriate parameters
Returns : none
Args : Hash ref with the mapped key (in %MAPPING) and value
charactersTitle : characters Usage : $eventgenerator->characters($str) Function: Send a character events Returns : none Args : string within_element Title : within_element
Usage : if( $eventgenerator->within_element($element) ) {}
Function: Test if we are within a particular element
This is different than 'in' because within can be tested
for a whole block.
Returns : boolean
Args : string element name
in_element Title : in_element
Usage : if( $eventgenerator->in_element($element) ) {}
Function: Test if we are in a particular element
This is different than 'within' because 'in' only
tests its immediate parent.
Returns : boolean
Args : string element name
start_documentTitle : start_document Usage : $eventgenerator->start_document Function: Handle a start document event Returns : none Args : none end_documentTitle : end_document Usage : $eventgenerator->end_document Function: Handles an end document event Returns : Bio::Search::Result::ResultI object Args : none result_countTitle : result_count Usage : my $count = $searchio->result_count Function: Returns the number of results we have processed Returns : integer Args : none model Title : model
Usage : my $model = $parser->model();
Function: Get/Set model; Infernal currently does not output
the model name (Rfam ID)
Returns : String (name of model)
Args : [optional] String (name of model)
database Title : database
Usage : my $database = $parser->database();
Function: Get/Set database; pre-v.1 versions of Infernal do not output
the database name
Returns : String (database name)
Args : [optional] String (database name)
algorithm Title : algorithm
Usage : my $algorithm = $parser->algorithm();
Function: Get/Set algorithm; pre-v.1 versions of Infernal do not output
the algorithm name
Returns : String (algorithm name)
Args : [optional] String (algorithm name)
query_accession Title : query_accession
Usage : my $acc = $parser->query_accession();
Function: Get/Set query (model) accession; pre-v1.1 Infernal does not output
the accession number (Rfam accession #)
Returns : String (accession)
Args : [optional] String (accession)
query_description Title : query_description
Usage : my $acc = $parser->query_description();
Function: Get/Set query (model) description; pre-v1.1 Infernal does not output
the Rfam description
Returns : String (description)
Args : [optional] String (description)
hsp_minscoreTitle : hsp_minscore Usage : my $cutoff = $parser->hsp_minscore(); Function: Get/Set min bit score cutoff (for generating Hits/HSPs) Returns : score (number) Args : [optional] score (number) convert_meta Title : convert_meta
Usage : $parser->convert_meta(1);
Function: Get/Set boolean flag for converting Infernal WUSS format
to a simple bracketed format (simple WUSS by default)
Returns : boolean flag (TRUE or FALSE)
Args : [optional] boolean (eval's to TRUE or FALSE)
versionTitle : version Usage : $parser->version(); Function: Set the Infernal cmsearch version Returns : version Args : [optional] version structure_symbols Title : structure_symbols
Usage : my $hashref = $parser->structure_symbols();
Function: Get/Set RNA structure symbols
Returns : Hash ref of delimiters (5' stem, 3' stem, single-strand, etc)
: default = < (5-prime)
> (3-prime)
: (single-strand)
? (unknown)
. (gap)
Args : Hash ref of substitute delimiters, using above keys.
simple_meta Title : simple_meta
Usage : my $string = $parser->simple_meta($str);
Function: converts more complex WUSS meta format into simple bracket format
using symbols defined in structure_symbols()
Returns : converted string
Args : [required] string to convert
Note : This is a very simple conversion method to get simple bracketed
format from Infernal data. If the convert_meta() flag is set,
this is the method used to convert the strings.
|