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Bio::Tools::Est2Genome(3) User Contributed Perl Documentation Bio::Tools::Est2Genome(3)

Bio::Tools::Est2Genome - Parse est2genome output, makes simple Bio::SeqFeature::Generic objects

  use Bio::Tools::Est2Genome;
  my $featureiter = Bio::Tools::Est2Genome->new(-file => 'output.est2genome');
  # This is going to be fixed to use the SeqAnalysisI next_feature
  # Method eventually when we have the objects to put the data in
  # properly
  while( my $f = $featureiter->parse_next_gene ) {
   # process Bio::SeqFeature::Generic objects here

This module is a parser for "est2genome" [EMBOSS] alignments of est/cdna sequence to genomic DNA. This is generally accepted as the best program for predicting splice sites based on est/dnas (as far as I know).
This module currently does not try pull out the ungapped alignments (Segment) but may in the future.

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.                  - General discussion  - About the mailing lists

Please direct usage questions or support issues to the mailing list:
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted the web:


The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

 Title   : new
 Usage   : my $obj = Bio::Tools::Est2Genome->new();
 Function: Builds a new Bio::Tools::Est2Genome object
 Returns : an instance of Bio::Tools::Est2Genome
 Args    : -file => 'output.est2genome' or
           -fh   => \*EST2GENOMEOUTPUT
           -genomefirst => 1  # genome was the first input (not standard)

 Usage     : $sim4->analysis_method();
 Purpose   : Inherited method. Overridden to ensure that the name matches
 Returns   : String
 Argument  : n/a

 Title   : parse_next_gene
 Usage   : @gene = $est2genome_result->parse_next_gene;
           foreach $exon (@exons) {
               # do something
 Function: Parses the next alignments of the est2genome result file and
           returns the found exons as an array of
           Bio::SeqFeature::SimilarityPair objects. Call
           this method repeatedly until an empty array is returned to get the
           results for all alignments.
           The $exon->seq_id() attribute will be set to the identifier of the
           respective sequence for both sequences.
           The length is accessible via the seqlength()
           attribute of $exon->query() and
 Returns : An array (or array reference) of Bio::SeqFeature::SimilarityPair and Bio::SeqFeature::Generic objects
           or Bio::SeqFeature::Gene::GeneStructure
 Args    : flag(1/0) indicating to return Bio::SeqFeature::Gene::GeneStructure or Bio::SeqFeature::SimilarityPair
           defaults to 0

 Title   : next_feature
 Usage   : $seqfeature = $obj->next_feature();
 Function: Returns the next feature available in the analysis result, or
           undef if there are no more features.
 Example :
 Returns : A Bio::SeqFeatureI implementing object, or undef if there are no
           more features.
 Args    : none
2019-01-01 perl v5.28.1

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